Anti-Human KIR3DL1/CD158E Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98389-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR3DL1/CD158E Rabbit RecAb (98389-1-RR, Clone:243022H8), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Human NCAM1/CD56. Cells were not fixed. Lymphocyte were gated.

• 

  FC experiment of human PBMCs using 98389-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR3DL1/CD158E Rabbit RecAb (98389-1-RR, Clone:243022H8)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocyte were gated.

KIR3DL1/CD158E Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98389-1-RR

Clone No.243022H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

KIR3DL1, CD158 antigen-like family member E, CD158E, CD158E1, HLA-BW4-specific inhibitory NK cell receptor

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98389-1-RR targets KIR3DL1/CD158E in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: NM_013289.4
• Gene Symbol: KIR3DL1
• Gene ID (NCBI): 3811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43629-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR3DL1 is a receptor on natural killer (NK) cells for HLA Bw4 allele. It inhibits the activity of NK cells thus preventing cell lysis. KIR3DL1 and KIR3DS1 are highly homologous.