KISS1 Recombinant monoclonal antibody, PBS Only

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• KISS1 Recombinant monoclonal antibody, PBS Only (88055-1-PBS)

KISS1 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 88055-1-PBS

Clone No.260303C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

• 

  WB analysis using 88055-1-RR (same clone as 88055-1-PBS)

  Human placenta tissue lysate was subjected to SDS PAGE followed by western blot with 88055-1-RR (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88055-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 88055-1-RR (same clone as 88055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88055-1-RR (KISS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88055-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 88055-1-RR (same clone as 88055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88055-1-RR (KISS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88055-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 88055-1-RR

  Biolayer interferometry (BLI) kinetic assays of 88055-1-RR against Human KISS1 were performed. The affinity constant is 12.3 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88055-1-PBS targets KISS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27198

  Product name: Recombinant human KISS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-138 aa of BC022819

  Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG

• Full Name: KiSS-1 metastasis-suppressor
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC022819
• Gene Symbol: KISS1
• Gene ID (NCBI): 3814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15726
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).