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KISS1R-Specific Polyclonal antibody, PBS Only (15505-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 15505-1-AP (same clone as 15505-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15505-1-AP (same clone as 15505-1-PBS)
Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 15505-1-AP (same clone as 15505-1-PBS)
Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

KISS1R-Specific Polyclonal antibody, PBS Only

Name: KISS1R-Specific Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 15505-1-PBS

KISS1R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15505-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15505-1-PBS targets KISS1R-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	KISS1 receptor
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	75 kDa, 43-45 kDa
GenBank Accession Number	NM_032551
Gene Symbol	KISS1R
Gene ID (NCBI)	84634
RRID	AB_10640578
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969F8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.