KLC2 Polyclonal antibody

KLC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17668-1-AP

Synonyms

kinesin light chain 2, KLC 2, KLC2



Tested Applications

Positive WB detected inmouse testis tissue, mouse lung tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17668-1-AP targets KLC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLC2 fusion protein Ag11158
Full Name kinesin light chain 2
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC034373
Gene Symbol KLC2
Gene ID (NCBI) 64837
RRIDAB_10597101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KLC2 antibody 17668-1-APDownload protocol
IHC protocol for KLC2 antibody 17668-1-APDownload protocol
IP protocol for KLC2 antibody 17668-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.

Authors - Yusuke Fukuda
humanWB

FASEB J

Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.

Authors - Amélie Robert
humanWB, IHC

Br J Cancer

High expression of kinesin light chain-2, a novel target of miR-125b, is associated with poor clinical outcome of elderly non-small-cell lung cancer patients.

Authors - M Wang
mouseWB

Mol Neurobiol

Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human.

Authors - Xiaolong Fu