KLC2 Polyclonal antibody

KLC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17668-1-AP

Synonyms

kinesin light chain 2, KLC 2, KLC2

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Validation Data Gallery

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  WB analysis of mouse testis using 17668-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17668-1-AP (KLC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17668-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17668-1-AP (KLC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 17668-1-AP

  IP result of anti-KLC2 (IP:17668-1-AP, 4ug; Detection:17668-1-AP 1:800) with mouse testis tissue lysate 4400ug.

• 

  IHC staining of human brain using 17668-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17668-1-AP (KLC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17668-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17668-1-AP (KLC2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse lung tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

17668-1-AP targets KLC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLC2 fusion protein Ag11158
• Full Name: kinesin light chain 2
• Calculated Molecular Weight: 622 aa, 69 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC034373
• Gene Symbol: KLC2
• Gene ID (NCBI): 64837
• RRID: AB_10597101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.; Authors - Yusuke Fukuda
human	WB	FASEB J; Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.; Authors - Amélie Robert
human	WB, IHC	Br J Cancer; High expression of kinesin light chain-2, a novel target of miR-125b, is associated with poor clinical outcome of elderly non-small-cell lung cancer patients.; Authors - M Wang
mouse	WB	Mol Neurobiol; Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human.; Authors - Xiaolong Fu