KLC2 Polyclonal antibody

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• KLC2 Polyclonal antibody (17668-1-AP)

KLC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17668-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

kinesin light chain 2, KLC 2, KLC2

• 

  WB analysis of mouse testis using 17668-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17668-1-AP (KLC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 17668-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17668-1-AP (KLC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 17668-1-AP

  IP result of anti-KLC2 (IP:17668-1-AP, 4ug; Detection:17668-1-AP 1:800) with mouse testis tissue lysate 4400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17668-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17668-1-AP (KLC2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17668-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17668-1-AP (KLC2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse lung tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17668-1-AP targets KLC2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11158

  Product name: Recombinant human KLC2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC034373

  Sequence: MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYA

• Full Name: kinesin light chain 2
• Calculated Molecular Weight: 622 aa, 69 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC034373
• Gene Symbol: KLC2
• Gene ID (NCBI): 64837
• RRID: AB_10597101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0B6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The KLC2 Polyclonal antibody (Cat# 17668-1-AP) has a total of 6 citations. These appear in NPJ Parkinson's Disease, British Journal of Cancer, Frontiers in Immunology, Journal of Cell Biology, Molecular Neurobiology, and FASEB Journal. Research fields span neurodegeneration (Parkinson's disease, axonal transport), oncology (non-small-cell lung cancer, retinoblastoma), neuroscience (axon survival, sensorineural hearing loss), and cell biology (intermediate filament transport, lysosomal trafficking).

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	NPJ Parkinsons Dis; Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling; Authors - Xiaoman Yang
human	WB, IHC	Br J Cancer; High expression of kinesin light chain-2, a novel target of miR-125b, is associated with poor clinical outcome of elderly non-small-cell lung cancer patients.; Authors - M Wang
human	WB	Front Immunol; RPL41 inhibits the proliferation and migration of retinoblastoma through the ARL5B-associated lysosomal trafficking.; Authors - Ye Li
human	WB	J Cell Biol; Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.; Authors - Yusuke Fukuda
mouse	WB	Mol Neurobiol; Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human.; Authors - Xiaolong Fu
human	WB	FASEB J; Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.; Authors - Amélie Robert