KLC4 Polyclonal antibody

KLC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16493-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Kinesin-like protein 8, KLC 4, KNSL8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 16493-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16493-1-AP (KLC4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat liver using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat pancreas using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 16493-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for KLC4 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	human small intestine tissue, human placenta tissue, human pancreas cancer tissue, human kidney tissue, rat pancreas tissue, rat liver tissue, mouse liver tissue, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16493-1-AP targets KLC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9621 Product name: Recombinant human KLC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC012357 Sequence: MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVLQSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVSIPCPPHPTPRTPHHCCFGLS Predict reactive species
Full Name	kinesin light chain 4
Calculated Molecular Weight	315 aa, 35 kDa
Observed Molecular Weight	69 kDa
GenBank Accession Number	BC012357
Gene Symbol	KLC4
Gene ID (NCBI)	89953
RRID	AB_2923563
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NSK0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.