KLF12 Polyclonal antibody

• 

  WB analysis using 13156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13156-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

KLF12 Polyclonal Antibody for WB, ELISA

Cat No. 13156-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ChIP, ELISA

Synonyms

AP 2rep, AP2REP, HSPC122, KLF12, Krueppel like factor 12, Kruppel like factor 12

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Tested Applications

• Positive WB detected in: HepG2 cells, human kidney tissue, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Product Information

13156-1-AP targets KLF12 in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3788

  Product name: Recombinant human KLF12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-402 aa of BC019680

  Sequence: MNIHMKRKTIKNINTFENRMLMLDGMPAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKGEPPEDSLSVDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTASASSPSSTSTSSSSSSRLASSPTVITSVSSASSSSTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRGHGKAQMDPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQKFPCSISPFSIESTRRQRRSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKFARSDELTRHYRKHTGVKPFKCADCDRSFSRSDHLALHRRRHMLV

• Full Name: Kruppel-like factor 12
• Calculated Molecular Weight: 402 aa, 44 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC019680
• Gene Symbol: KLF12
• Gene ID (NCBI): 11278
• RRID: AB_2132260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4X4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF12, also named as AP2REP or HSPC122, is a 402 amino acid protein, which contains three C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. KLF12 localizes in the nucleus and confers strong transcriptional repression to the AP-2-alpha gene. KLF12 is atranscriptional silencer to the AP2-alpha gene, mediating positive and negative regulation of the AP2-A expression trhough a set of overlapping cis-regulatory promoters elements.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; Tumour-suppression function of KLF12 through regulation of anoikis.; Authors - N Godin-Heymann ; KD Validated
human	WB	Front Oncol; circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma.; Authors - Bing Guan
mouse	WB	J Ethnopharmacol; Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.; Authors - Jing Zhou
human	WB	Onco Targets Ther; The role of miR-382-5p in glioma cell proliferation, migration and invasion.; Authors - Jinjin Wang
human	WB,IHC	Biochem Biophys Res Commun; Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization.; Authors - Shen Xiaoyue X
mouse	WB	Genes Dis; All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members.; Authors - Jiejie Cui