Tested Applications
| Positive WB detected in | HepG2 cells, human kidney tissue, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13156-1-AP targets KLF12 in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3788 Product name: Recombinant human KLF12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC019680 Sequence: MNIHMKRKTIKNINTFENRMLMLDGMPAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKGEPPEDSLSVDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTASASSPSSTSTSSSSSSRLASSPTVITSVSSASSSSTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRGHGKAQMDPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQKFPCSISPFSIESTRRQRRSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKFARSDELTRHYRKHTGVKPFKCADCDRSFSRSDHLALHRRRHMLV Predict reactive species |
| Full Name | Kruppel-like factor 12 |
| Calculated Molecular Weight | 402 aa, 44 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC019680 |
| Gene Symbol | KLF12 |
| Gene ID (NCBI) | 11278 |
| RRID | AB_2132260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4X4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KLF12, also named as AP2REP or HSPC122, is a 402 amino acid protein, which contains three C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. KLF12 localizes in the nucleus and confers strong transcriptional repression to the AP-2-alpha gene. KLF12 is atranscriptional silencer to the AP2-alpha gene, mediating positive and negative regulation of the AP2-A expression trhough a set of overlapping cis-regulatory promoters elements.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF12 antibody 13156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KLF12 antibody (Cat# 13156-1-AP) has 10 citations across journals including Oncogene, Cell Reports, Genes Discovery, Molecular Oncology, Frontiers in Oncology, International Journal of Biological Macromolecules, Journal of Ethnopharmacology, BMC Veterinary Research, Biochemical and Biophysical Research Communications, and OncoTargets and Therapy. Research fields span hepatocarcinoma, pancreatic cancer, lung cancer, breast cancer, glioma, CAR-T cell therapy, vascular smooth muscle biology, ovarian granulosa cell function, endometrial decidualization, and miRNA-mediated gene regulation.
| Species | Application | Title |
|---|---|---|
Genes Dis All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. | ||
Int J Biol Macromol LINC01137 facilitates pancreatic cancer stemness, chemoresistance and proliferation via the miR-7155-5p/KLF12/PI3K-Akt axis. | ||
Cell Rep Class I HDAC inhibitors enhance antitumor efficacy and persistence of CAR-T cells by activation of the Wnt pathway | ||
J Ethnopharmacol Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose. | ||
Mol Oncol KLF12 transcriptionally regulates PD-L1 expression in non-small cell lung cancer
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