KLF5 Monoclonal antibody

• Featured Product
• KD/KO Validated

KLF5 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C11C5

Cat no : 66850-1-Ig

Synonyms

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

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Validation Data Gallery

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  WB analysis of HeLa using 66850-1-Ig

  WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells.

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  WB analysis using 66850-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 66850-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

Sample-dependent, check data in validation data gallery

Product Information

66850-1-Ig targets KLF5 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLF5 fusion protein Ag15403
• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated molecular weight: 457 aa, 51 kDa
• Observed molecular weight: 51-57 kDa
• GenBank accession number: BC042131
• Gene symbol: KLF5
• Gene ID (NCBI): 688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.