KLHL11 Polyclonal antibody

KLHL11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17117-1-AP

Synonyms

kelch like 11 (Drosophila), Kelch like protein 11, KLHL11

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Validation Data Gallery

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  WB analysis of COLO 320 using 17117-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17117-1-AP (KLHL11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17117-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17117-1-AP (KLHL11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17117-1-AP targets KLHL11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL11 fusion protein Ag10805
• Full Name: kelch-like 11 (Drosophila)
• Calculated Molecular Weight: 708 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC034470
• Gene Symbol: KLHL11
• Gene ID (NCBI): 55175
• RRID: AB_2130733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL11 is a component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating ubiquitination of target proteins. The presence of antibodies directed against KLHL11 has been identified as biomarker of a paraneoplastic neurological syndrome. The downregulation of KLHL11 may contribute to the disease progression, owing to its putative function in protein quality control. The calculated molecular weight of KLHL11 is 80 kDa (PMID: 34312900).