KLHL20 Polyclonal antibody

KLHL20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18111-1-AP

Synonyms

kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3

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Validation Data Gallery

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  WB analysis of HEK-293 using 18111-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18111-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18111-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18111-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18111-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18111-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18111-1-AP is KLHL20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL20 fusion protein Ag12567
• Full Name: kelch-like 20 (Drosophila)
• Calculated Molecular Weight: 609 aa, 68 kDa
• Observed Molecular Weight: 65-67 kDa
• GenBank Accession Number: BC063418
• Gene Symbol: KLHL20
• Gene ID (NCBI): 27252
• RRID: AB_2131189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.