A431 cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16952-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16952-1-AP
IP result of anti-KLHL21 (IP:16952-1-AP, 3ug; Detection:16952-1-AP 1:300) with HeLa cells lysate 1800ug.
IP result of anti-KLHL21 (IP:16952-1-AP, 3ug; Detection:16952-1-AP 1:300) with HeLa cells lysate 1800ug.
IHC staining of human liver cancer using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
A431 cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16952-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 16952-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of HeLa using 16952-1-AP
IP result of anti-KLHL21 (IP:16952-1-AP, 3ug; Detection:16952-1-AP 1:300) with HeLa cells lysate 1800ug.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
KD/KO VALIDATED
KLHL21 Polyclonal antibody
Catalog Number
16952-1-AP
Citations
2
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200
Applications
WB, IP, IHC, ELISA
Reactivity
human, mouse, rat, oryctolagus cuniculus (rabbit)
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
