KLHL22 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLHL22 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 16214-1-AP

Synonyms

kelch like 22 (Drosophila), Kelch like protein 22, KELCHL, KLHL22

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Validation Data Gallery

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  WB analysis using 16214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16214-1-AP (KLHL22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 16214-1-AP

  IP result of anti-KLHL22 (IP:16214-1-AP, 4ug; Detection:16214-1-AP 1:500) with PC-3 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IP: See 2 publications below

Product Information

16214-1-AP targets KLHL22 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL22 fusion protein Ag9212
• Full Name: kelch-like 22 (Drosophila)
• Calculated Molecular Weight: 634 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC015923
• Gene Symbol: KLHL22
• Gene ID (NCBI): 84861
• RRID: AB_2131201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.; Authors - Jie Chen ; KD Validated
human	WB	J Cell Biol; Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.; Authors - Xiaolong Zhuo ; KD Validated
human	WB	Cell Rep; SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.; Authors - Donghua Wen ; KD Validated
mouse	WB	iScience; Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke; Authors - Shuang Jin
human	WB	J Hum Genet; A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders.; Authors - Kohji Kato
human	WB	Neoplasma; KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.; Authors - X R Liu