Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16214-1-AP targets KLHL22 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9212 Product name: Recombinant human KLHL22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 81-634 aa of BC015923 Sequence: GGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCCDFLMSWVDEENILDVYRLAELFDLSRLTEQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQISLHEPPKLLETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNESLQPSLQSPQTELRSDFQCVVGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNFVYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLSVCVVGRYIYAVAGRDYHNDLNAVERYDPATNSWAYVAPLKREVYAHAGATLEGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMATLLNKLYVIGGSNNDAGYRRDVHQVACYSCTSGQWSSVCPLPAGHGEPGIAVLDNRIYVLGGRSHNRGSRTGYVHIYDVEKDCWEEGPQLDNSISGLAACVLTLPRSLLLEPPRGTPDRSQADPDFASEVMSVSDWEEFDNSSED Predict reactive species |
| Full Name | kelch-like 22 (Drosophila) |
| Calculated Molecular Weight | 634 aa, 72 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC015923 |
| Gene Symbol | KLHL22 |
| Gene ID (NCBI) | 84861 |
| RRID | AB_2131201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53GT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for KLHL22 antibody 16214-1-AP | Download protocol |
| WB protocol for KLHL22 antibody 16214-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KLHL22 antibody (Cat# 16214-1-AP) has 14 citations spanning 11 journals including Nature, Nature Communications, Cell Reports, EMBO Reports, Journal of Cell Biology, Biochemical Pharmacology, Cell Oncology, iScience, Development, Neoplasma, and Journal of Human Genetics. Research fields covered include mTORC1 signaling, E3 ubiquitin ligase function, mitotic regulation, hepatocellular carcinoma, melanoma, brain development, stroke recovery, and viral interferon responses. Applications are primarily Western blot and immunoprecipitation in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nature KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.
| ||
Nat Commun A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3KLHL22 | ||
Cell Rep SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.
| ||
Cell Rep The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation | ||
Biochem Pharmacol UBE4B modulates BET inhibitor sensitivity via KLHL22-JAK2-PIM1 axis in hepatocellular carcinoma |
