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What's the best dilution for KLHL9 in ?
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55394-1-AP targets KLHL9 in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | kelch-like 9 (Drosophila) |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | NM_018847 |
| Gene Symbol | KLHL9 |
| Gene ID (NCBI) | 55958 |
| RRID | AB_2881320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2J3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLHL9 antibody 55394-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KLHL9 Polyclonal antibody (Cat# 55394-1-AP) has 1 total citation. It appears in Cell Reports (2024), where it was used for WB, IP, and IF in human and mouse samples. The research field involves the Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex, Rheb ubiquitination, and amino acid-induced mTORC1 activation.
| Species | Application | Title |
|---|---|---|
Cell Rep The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation |
