Anti-Human KLRB1/CD161 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human peripheral blood leukocytes using 98281-1-RR (same clone as 98281-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD161/KLRB1 Rabbit RecAb (98281-1-RR, Clone: 242140H5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98281-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98281-1-RR (same clone as 98281-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD161/KLRB1 Rabbit RecAb (98281-1-RR, Clone:242140H5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98281-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98281-1-RR (same clone as 98281-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD161/KLRB1 Rabbit RecAb (98281-1-RR, Clone: 242140H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98281-1-PBS in a different storage buffer formulation.

KLRB1/CD161 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98281-1-PBS

Clone No.242140H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD161, 242140H5, HNKR-P1a, hNKR P1A, C-type lectin domain family 5 member B

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Product Information

98281-1-PBS targets KLRB1/CD161 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: killer cell lectin-like receptor subfamily B, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_002258.3
• Gene Symbol: CD161
• Gene ID (NCBI): 3820
• ENSEMBL Gene ID: ENSG00000111796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q12918
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).