KLRF1 Polyclonal antibody

KLRF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21510-1-AP

Synonyms

Activating coreceptor NKp80, CLEC5C, KLRF1, Lectin like receptor F1, ML, NKp80

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Validation Data Gallery

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  WB analysis of human spleen using 21510-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 21510-1-AP (KLRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21510-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 21510-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21510-1-AP is KLRF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLRF1 fusion protein Ag16008
• Full Name: killer cell lectin-like receptor subfamily F, member 1
• Calculated molecular weight: 231 aa, 27 kDa
• Observed molecular weight: 68 kDa
• GenBank accession number: BC098257
• Gene symbol: KLRF1
• Gene ID (NCBI): 51348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.