Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13963-1-AP targets KPNA5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4920 Product name: Recombinant human KPNA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC047409 Sequence: MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTR Predict reactive species |
| Full Name | karyopherin alpha 5 (importin alpha 6) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC047409 |
| Gene Symbol | KPNA5 |
| Gene ID (NCBI) | 3841 |
| RRID | AB_2133847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15131 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KPNA5 encodes karyopherin subunit alpha 5, an importin alpha adapter protein that mediates nuclear protein import by recognizing classical and non-classical nuclear localization signals. It is part of the machinery that carries selected proteins from the cytoplasm to the nucleus and supports signal-dependent nuclear trafficking. Its expression is high in the male reproductive system and is also detected in brain, eye, and renal system tissues. KPNA5 acts with KPNB1 to dock cargo at the nuclear pore and enable Ran-dependent translocation into the nucleus. Through this transport route, it recognizes nuclear localization motifs in transcription factors and viral proteins, including STAT1, STAT2, and influenza nucleoprotein, thereby linking cargo recognition at the cytosol to import across the nuclear pore complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for KPNA5 antibody 13963-1-AP | Download protocol |
| WB protocol for KPNA5 antibody 13963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. | ||
Virol Sin African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. | ||
J Immunol Cutting edge: tubulin α functions as an adaptor in NFAT-importin β interaction. | ||
PLoS One An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. | ||
J Neurosci miR-153 prevents NRF2 nuclear translocation to drive hypoperfusion-related cognitive deficits by targeting KPNA5 | ||
