Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, human cerebellum tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16094-1-AP targets KPTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8785 Product name: Recombinant human KPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 79-436 aa of BC009249 Sequence: EIVSIDTFNKSPPKRGLVVGITFIKDSGDKGSPFLNIYCDYEPGSEYNLDSIAQSCLNLELQFTPFQLCHAEVQVGDQLETVFLLSGNDPAIHLYKENEGLHQFEEQPVENLFPELTNLTSSVLWLDVHNFPGTSRRLSALGCQSGYVRVAHVDQRSREVLQMWSVLQDGPISRVIVFSLSAAKETKDRPLQDEYSVLVASMLEPAVVYRDLLNRGLEDQLLLPGSDQFDSVLCSLVTDVDLDGRPEVLVATYGQELLCYKYRGPESGLPEAQHGFHLLWQRSFSSPLLAMAHVDLTGDGLQELAVVSLKGVHILQHSLIQASELVLTRLRHQVEQRRRRLQGLEDGAGAGPAENAAS Predict reactive species |
| Full Name | kaptin (actin binding protein) |
| Calculated Molecular Weight | 436 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC009249 |
| Gene Symbol | KPTN |
| Gene ID (NCBI) | 11133 |
| RRID | AB_2134007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y664 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KPTN antibody 16094-1-AP | Download protocol |
| IHC protocol for KPTN antibody 16094-1-AP | Download protocol |
| WB protocol for KPTN antibody 16094-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-KPTN antibody (Cat# 16094-1-AP) has 13 total citations spanning journals including Nature (×4), Science, Nature Metabolism, Nature Cell Biology, Nature Communications, JCI, PNAS, Cell Reports, Cells, and JBC. Research fields center on mTORC1 nutrient sensing, lysosomal GATOR1/GATOR2 complex architecture, amino acid and glucose signaling, and tumor growth regulation. All applications were Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Nature KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
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