Back

Cytokeratin 14 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 22221-1-AP

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 26 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more
Tested Applications
Applications:
ELISA, WB, IP, IHC, IF
Application not tested? Please see our Guarantee


Images See all

Cytokeratin 14 Antibody WB mouse skin tissue 22221-1-AP

mouse skin tissue were subjected to SDS PAGE followed by western blot with 22221-1-AP(Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Cytokeratin 14 Antibody IHC human skin tissue 22221-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 22221-1-AP( Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

Cytokeratin 14 Antibody IHC human skin tissue 22221-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 22221-1-AP( Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

Cytokeratin 14 Antibody IF HepG2 cells 22221-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 22221-1-AP(Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 14 Antibody IP mouse skin tissue 22221-1-AP

IP Result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

Species specificity:
human, mouse, hamster
Positive WB detected in:
mouse skin tissue
Positive IP detected in:
mouse skin tissue
Positive IHC detected in:
human skin tissue, human breast cancer tissue, human cervical cancer tissue, human lung cancer tissue, human skin tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 14 fusion protein ag17559
Full name:
keratin 14
Calculated molecular weight:
472aa,52 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC002690
Gene ID (NCBI):
3861
Gene symbol
KRT14
Synonyms
CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ
Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically agianst KRT14.


Back
to top