Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22221-1-AP

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Validation Data Gallery

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  WB analysis of mouse skin using 22221-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 22221-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IP experiment of mouse skin using 22221-1-AP

  IP Result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  FC experiment of A431 using 22221-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 14 (22221-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human skin tissue, human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 22221-1-AP is Cytokeratin 14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: hamster, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Full Name: keratin 14
• Calculated molecular weight: 472 aa, 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC002690
• Gene symbol: KRT14
• Gene ID (NCBI): 3861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.

Publications

Species	Application	Title
human, hamster	WB	Cancer Gene Ther; Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.; Authors - S Vijayalingam