Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22221-1-AP

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ



Tested Applications

Positive WB detected inmouse skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue, human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22221-1-AP is Cytokeratin 14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity hamster, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag17559
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IHC protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IF protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IP protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, hamsterWB

Cancer Gene Ther

Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.

Authors - S Vijayalingam