Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse skin using 22221-1-AP
IP result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.
IP result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.
IHC staining of human skin using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human bowen disease using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human cervical cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cervical cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human breast cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IF Staining of mouse skin using 22221-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human skin tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse skin tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.
Protocols
Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 22221-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human skin using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human bowen disease using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human cervical cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cervical cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human breast cancer using 22221-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
IP Figures
IP experiment of mouse skin using 22221-1-AP
IP result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.
IF-P Figures
IF Staining of mouse skin using 22221-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skin using 22221-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Cytokeratin 14 Polyclonal antibody
Catalog Number
22221-1-AP
Citations
3
Dilutions
WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat, monkey, hamster
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
