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Cytokeratin 14 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 22221-1-AP

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Con: 26 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Cytokeratin 14 Antibody WB mouse skin tissue 22221-1-AP

mouse skin tissue were subjected to SDS PAGE followed by western blot with 22221-1-AP(Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Cytokeratin 14 Antibody IHC human skin tissue 22221-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 22221-1-AP( Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

Cytokeratin 14 Antibody IHC human skin tissue 22221-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 22221-1-AP( Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

Cytokeratin 14 Antibody IF HepG2 cells 22221-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 22221-1-AP(Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 14 Antibody IP mouse skin tissue 22221-1-AP

IP Result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

Species specificity:
human, mouse, hamster
Positive WB detected in:
mouse skin tissue
Positive IP detected in:
mouse skin tissue
Positive IHC detected in:
human skin tissue, human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 14 fusion protein Ag17559
Full name:
keratin 14
Calculated molecular weight:
472aa,52 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC002690
Gene ID (NCBI):
3861
Gene symbol
KRT14
Synonyms
CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.


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