Validation Data Gallery
|Positive WB detected in||HeLa cells, A431 cells, K-562 cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, human colon cancer tissue, human ovary tumor tissue, human endometrial cancer tissue, human kidney tissue, human lung cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells, mouse olfactory epithelium tissue|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10830-1-AP targets Cytokeratin 18 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bull, calves, cow, duck, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 18 fusion protein Ag1260|
|Full Name||keratin 18|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC000180|
|Gene ID (NCBI)||3875|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
|Product Specific Protocols|
|WB protocol for Cytokeratin 18 antibody 10830-1-AP||Download protocol|
|IHC protocol for Cytokeratin 18 antibody 10830-1-AP||Download protocol|
|IF protocol for Cytokeratin 18 antibody 10830-1-AP||Download protocol|
|IP protocol for Cytokeratin 18 antibody 10830-1-AP||Download protocol|
|FC protocol for Cytokeratin 18 antibody 10830-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Med
Wnt5a induces and maintains prostate cancer cells dormancy in bone.
Cytokeratin expression in epidermal stem cells in skin adnexal tumors.
Thyroid hormone (T3) is involved in inhibiting the proliferation of newborn calf Sertoli cells via the PI3K/Akt signaling pathway in vitro.
Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation.
J Oral Biosci
PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner.
Exp Lung Res
Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine model of allergic asthma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Parmveer (Verified Customer) (12-02-2019)
Works well for both IHC and IF, but does come with a bit of background