Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10830-1-AP

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18



Tested Applications

Positive WB detected inHeLa cells, A431 cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, human colon cancer tissue, human ovary tumor tissue, human endometrial cancer tissue, human kidney tissue, human lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells, mouse olfactory epithelium tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10830-1-AP targets Cytokeratin 18 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine, duck, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol KRT18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IHC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IF protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IP protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
mouseIF

Hepatology

Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.

Authors - Bing Han
mouseIHC,IF

Proc Natl Acad Sci U S A

Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.

Authors - Wada-Hiraike Osamu O
mouseIF

Proc Natl Acad Sci U S A

Basal epithelial stem cells are efficient targets for prostate cancer initiation.

Authors - Lawson Devon A DA
humanWB

Oncogene

EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.

Authors - C-F Yang
mouseIF,FC

Kidney Int

Carbon monoxide potently prevents ischemia-induced high-mobility group box 1 translocation and release and protects against lethal renal ischemia-reperfusion injury.

Authors - Yongle Ruan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)