CoraLite®594-conjugated Cytokeratin 18 Polyclonal antibody

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  WB analysis using CL594-10830

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-10830 (Cytokeratin 18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-10830

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Cytokeratin 18 antibody (CL594-10830) at dilution of 1:200.

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  IF Staining of HeLa using CL594-10830

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytokeratin 18 antibody (CL594-10830) at dilution of 1:200.

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  FC experiment of HeLa using CL594-10830

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Cytokeratin 18 (CL594-10830) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 18 Polyclonal Antibody for WB, IF/ICC, FC (Intra)

Cat No. CL594-10830

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra)

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10830 targets Cytokeratin 18 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1260

  Product name: Recombinant human KRT18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 127-430 aa of BC000180

  Sequence: SHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

• Full Name: keratin 18
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000180
• Gene Symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• RRID: AB_2919747
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05783
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.