Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10830-1-AP (same clone as 10830-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
WB analysis using 10830-1-AP (same clone as 10830-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10830-1-AP (same clone as 10830-1-PBS)
WB result of Cytokeratin 18 antibody (10830-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IP experiment of A431 using 10830-1-AP (same clone as 10830-1-PBS)
IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10830-1-AP (same clone as 10830-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 10830-1-AP (same clone as 10830-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 10830-1-AP (same clone as 10830-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Cytokeratin 18 Polyclonal antibody, PBS Only

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10830-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10830-1-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1260 Product name: Recombinant human KRT18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 127-430 aa of BC000180 Sequence: SHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH Predict reactive species
Full Name	keratin 18
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC000180
Gene Symbol	Cytokeratin 18
Gene ID (NCBI)	3875
RRID	AB_2133164
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05783
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.