Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 66187-1-Ig

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18



Tested Applications

Positive WB detected inHCT 116 cells, pig skin tissue, A431 cells, HeLa cells, HepG2 cells, COLO 320 cells, K-562 cells, 4T1 cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human renal cell carcinoma tissue, human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, mouse liver tissue, rat liver tissue, mouse kidney tissue, human tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:600-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66187-1-Ig targets Cytokeratin 18 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol KRT18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
IHC protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
IF protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
FC protocol for Cytokeratin 18 antibody 66187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,IHC

Commun Biol

Single-cell analysis supports a luminal-neuroendocrine transdifferentiation in human prostate cancer.

Authors - Baijun Dong
humanWB

Hum Reprod

T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis.

Authors - Qinsheng Lu
humanIF

J Pathol

Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.

Authors - Qiuli Liu