|Positive WB detected in||HCT 116 cells, pig skin tissue, A431 cells, HeLa cells, HepG2 cells, COLO 320 cells, K-562 cells, 4T1 cells|
|Positive IHC detected in||human kidney tissue, human liver tissue, human renal cell carcinoma tissue, human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, mouse liver tissue, rat liver tissue, mouse kidney tissue, human tonsillitis tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunohistochemistry (IHC)||IHC : 1:600-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66187-1-Ig targets Cytokeratin 18 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratin 18 fusion protein Ag1260|
|Full Name||keratin 18|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC000180|
|Gene ID (NCBI)||3875|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
|Product Specific Protocols|
|WB protocol for Cytokeratin 18 antibody 66187-1-Ig||Download protocol|
|IHC protocol for Cytokeratin 18 antibody 66187-1-Ig||Download protocol|
|IF protocol for Cytokeratin 18 antibody 66187-1-Ig||Download protocol|
|FC protocol for Cytokeratin 18 antibody 66187-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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