CoraLite® Plus 488-conjugated Cytokeratin 18 Monoclonal antibody

Name: CoraLite® Plus 488-conjugated Cytokeratin 18 Monoclonal antibody, 100ul
Brand: Proteintech
SKU: CL488-66187-100UL

Cytokeratin 18 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66187

Clone No.1G11C4

Publications(1)

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, canine

Applications

IF/ICC, FC (Intra)

Synonyms

KRT18, 1G11C4, Cell proliferation-inducing gene 46 protein, CK 18, CK18

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite® Plus 488

Unconjugated CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®532 CoraLite®555 CoraLite®594 FITC

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Filter: AllIF/ICCFC (Intra)

IF Staining of HeLa using CL488-66187
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MDCK using CL488-66187
Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using CL488-66187
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using CL488-66187
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using CL488-66187
1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Human Cytokeratin 18 (CL488-66187, Clone:1G11C4) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IF/ICC detected in	HeLa cells, HepG2 cells, MDCK cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66187 targets Cytokeratin 18 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

Tested Reactivity	human, canine
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1260 Product name: Recombinant human KRT18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-430 aa of BC000180 Sequence: HYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH Predict reactive species
Full Name	keratin 18
Calculated Molecular Weight	48 kDa
GenBank Accession Number	BC000180
Gene Symbol	Cytokeratin 18
Gene ID (NCBI)	3875
RRID	AB_2883278
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	493 nm / 522 nm
Excitation Laser	Blue laser (488 nm)
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P05783
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187	Download protocol
IF protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187	Download protocol