Tested Applications
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, MDCK cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66187 targets Cytokeratin 18 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1260 Product name: Recombinant human KRT18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-430 aa of BC000180 Sequence: HYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH Predict reactive species |
| Full Name | keratin 18 |
| Calculated Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000180 |
| Gene Symbol | Cytokeratin 18 |
| Gene ID (NCBI) | 3875 |
| RRID | AB_2883278 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05783 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187 | Download protocol |
| IF protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CoraLite® Plus 488-conjugated Cytokeratin 18 Monoclonal antibody (Cat# CL488-66187) has 1 citation to date. The citation appears in Nature Communications (Impact Factor 18.1), a 2025 study investigating lactate mitochondrial oxidation and its role in driving stemness potential in metastatic breast cancer. The antibody was applied in immunofluorescence (IF) on human samples. The associated research field encompasses breast cancer biology, cancer stemness, and metabolic reprogramming.
| Species | Application | Title |
|---|---|---|
Nat Commun Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer. |
