Cytokeratin 23 Polyclonal antibody

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  WB analysis of BxPC-3 using 24049-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24049-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24049-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 24049-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 23 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24049-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

KRT23, CK 23, CK23, CK-23, Cytokeratin-23

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

24049-1-AP targets Cytokeratin 23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21129

  Product name: Recombinant human KRT23 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-422 aa of BC028356

  Sequence: MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVHGGAGGARISLSFTTRSCPPPGGSWGSGRSSPLLGGNGKATMQNLNDRLASYVEKVRALEEANMKLESRILKWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTNAQIILLIDNARMAVDDFNLKYENEHSFKKDLEIEVEGLRRTLDNLTIVTTDLEQEVEGMRKELILMKKHHEQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMRQEYELIIKKKHRDLDTWYKEQSAAMSQEAASPATVQSRQGDIHELKRTFQALEIDLQTQYSTKSALENMLSETQSRYSCKLQDMQEIISHYEEELTQLRHELERQNNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREESKSSMKVFATPKIKAITQETINGRLVLCQVNEIQKHA

• Full Name: keratin 23 (histone deacetylase inducible)
• Calculated Molecular Weight: 422 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC028356
• Gene Symbol: KRT23
• Gene ID (NCBI): 25984
• RRID: AB_2879416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C075
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Rep; Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model.; Authors - Masatoshi Ohgushi