Cytokeratin 36 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Cytokeratin 36 Polyclonal antibody (14309-1-AP)

Cytokeratin 36 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14309-1-AP Copied!

Publications (4)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

keratin 36, KRT36, CK36, CK-36, keratin36

• 

  WB analysis of mouse skin using 14309-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 14309-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Cytokeratin 36 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14309-1-AP targets Cytokeratin 36 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5483

  Product name: Recombinant human KRT36 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-417 aa of BC043581

  Sequence: MLRSGLSGLGSCLPGSYLSSECHTSGFVGSGGWFCEGSFNGSEKETMQFLNDRLANYLEKVRQLERENAELESRIQEWYEFQIPYICPDYQSYFKTIEDFQQKILLTKSENARLVLQIDNAKLAADDFRTKYETELSLRQLVEADINGLRRILDELTLCKADLEAQVESLKEELMCLKKNHEEEVSVLRCQLGDRLNVEVDAAPPVDLNKILEDMRCQYEALVENNRRDVEAWFNTQTEELNQQVVSSSEQLQCCQTEIIELRRTVNALEIELQAQHSMRNSLESTLAETEARYSSQLAQMQCLISNVEAQLSEIRCDLERQNQEYQVLLDVKARLEGEIATYRHLLEGEDCKLPPQPCATACKPVIRVPSVPPVPCVPSVPCTPAPQVGTQIRTITEEIRDGKVISSREHVQSRPL

• Full Name: keratin 36
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC043581
• Gene Symbol: KRT36
• Gene ID (NCBI): 8689
• RRID: AB_2134018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O76013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Cytokeratin 36 Polyclonal antibody (Cat# 14309-1-AP) has a total of 4 citations. These appear in Aging Cell, Journal of Investigative Dermatology (2 papers), and eLife. The associated research fields include epidermal stem cell heterogeneity during skin aging, spatial organization of epithelial structures in skin and oral mucosa, retinoic acid signaling in epidermal stem cell populations, and Hedgehog signaling in tongue development. All cited applications were immunofluorescence (IF) in mouse or human samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Aging Cell; Integrin-Binding Matricellular Protein Fibulin-5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging.; Authors - Wenxin Fan
mouse	IF	J Invest Dermatol; Retinoic Acid Signaling Alters the Balance of Epidermal Stem Cell Populations in the Skin.; Authors - Thisakorn Dumrongphuttidecha
mouse	IF	J Invest Dermatol; Spatial organization of epithelial heterogeneity through undulating structures of the skin and oral mucosa.; Authors - Mizuho Ishikawa
human	IF	Elife; Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling; Authors - Maiko Kawasaki