Cytokeratin 36 Polyclonal antibody

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  WB analysis of mouse skin using 14309-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 14309-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 14309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

Cytokeratin 36 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14309-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

keratin 36, KRT36, CK36, CK-36, keratin36

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

14309-1-AP targets Cytokeratin 36 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5483

  Product name: Recombinant human KRT36 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-417 aa of BC043581

  Sequence: MLRSGLSGLGSCLPGSYLSSECHTSGFVGSGGWFCEGSFNGSEKETMQFLNDRLANYLEKVRQLERENAELESRIQEWYEFQIPYICPDYQSYFKTIEDFQQKILLTKSENARLVLQIDNAKLAADDFRTKYETELSLRQLVEADINGLRRILDELTLCKADLEAQVESLKEELMCLKKNHEEEVSVLRCQLGDRLNVEVDAAPPVDLNKILEDMRCQYEALVENNRRDVEAWFNTQTEELNQQVVSSSEQLQCCQTEIIELRRTVNALEIELQAQHSMRNSLESTLAETEARYSSQLAQMQCLISNVEAQLSEIRCDLERQNQEYQVLLDVKARLEGEIATYRHLLEGEDCKLPPQPCATACKPVIRVPSVPPVPCVPSVPCTPAPQVGTQIRTITEEIRDGKVISSREHVQSRPL

• Full Name: keratin 36
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC043581
• Gene Symbol: KRT36
• Gene ID (NCBI): 8689
• RRID: AB_2134018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O76013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Elife; Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling; Authors - Maiko Kawasaki