Cytokeratin 78 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of mouse skin using 86328-1-RR (same clone as 86328-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 86328-1-RR (KRT78 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86328-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skin using 86328-1-RR (same clone as 86328-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 86328-1-RR (KRT78 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 86328-1-RR (same clone as 86328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86328-1-RR (Cytokeratin 78 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86328-1-PBS in a different storage buffer formulation.

Cytokeratin 78 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86328-1-PBS

Clone No.250889G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CK-78, Cytokeratin-78, K5B, K78, KB40

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Product Information

86328-1-PBS targets Cytokeratin 78 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 78
• Calculated Molecular Weight: 56kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_001300814
• Gene Symbol: Cytokeratin 78
• Gene ID (NCBI): 196374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N1N4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The gene of KRT78 is a member of the type II keratin family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses.