Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10723-1-AP targets KU70/XRCC6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1043 Product name: Recombinant human KU70,XRCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 307-609 aa of BC008343 Sequence: TGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD Predict reactive species |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC008343 |
| Gene Symbol | KU70 |
| Gene ID (NCBI) | 2547 |
| RRID | AB_2218756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12956 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IF protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IHC protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IP protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| WB protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (catalog no. 10723-1-AP) has 55 citations spanning journals including Nature Communications, Cancer Research, Molecular Cell, Journal of Clinical Investigation, Cell Death & Disease, Oncogene, Genome Biology, FASEB Journal, and Journal of Biological Chemistry, among others. Research fields center on DNA damage repair (NHEJ/HR), cancer biology (lung, liver, colorectal, breast, ovarian, glioma, pancreatic), radio/chemoresistance, DNA damage response signaling, and related topics such as alternative splicing, lipid metabolism, and immunology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma | ||
Cancer Res DNA-PK-Mediated Cytoplasmic DNA Sensing Stimulates Glycolysis to Promote Lung Squamous Cell Carcinoma Malignancy and Chemoresistance | ||
Drug Resist Updat Targeting CLK1/SRSF7 axis-dependent alternative splicing sensitizes pancreatic ductal adenocarcinoma to chemotherapy and immunotherapy | ||
Nat Commun Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining. | ||
Nat Commun Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis |
Reviews
What customers say
The single review for 10723-1-AP is a 5-star rating for Western Blotting. The reviewer reported that the antibody worked well with little background, indicating strong specificity and clean results. Overall, the feedback is positive, highlighting reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jonathan (Verified Customer) (02-03-2019) | Worked well for Western Blotting with little background.
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