KU70,XRCC6 Monoclonal antibody
KU70,XRCC6 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3C12D9
Cat no : 66607-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human breast cancer tissue, HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
66607-1-Ig targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | KU70,XRCC6 fusion protein Ag21301 |
Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
Calculated molecular weight | 75 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC008343 |
Gene symbol | XRCC6 |
Gene ID (NCBI) | 2547 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
IHC protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
IF protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. | ||
Cancer Lett ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer | ||
J Nanobiotechnology Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles. | ||
Cell Rep circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells |