KU70,XRCC6 Monoclonal antibody

KU70,XRCC6 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3C12D9

Cat no : 66607-1-Ig

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6

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Validation Data Gallery

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  WB analysis using 66607-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66607-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66607-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66607-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66607-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human lung cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66607-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66607-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66607-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of human breast cancer using 66607-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66607-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human KU70,XRCC6 (66607-1-Ig, Clone:3C12D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, human breast cancer tissue, HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

66607-1-Ig targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KU70,XRCC6 fusion protein Ag21301
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 6
• Calculated molecular weight: 75 kDa
• Observed molecular weight: 70 kDa
• GenBank accession number: BC008343
• Gene symbol: XRCC6
• Gene ID (NCBI): 2547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Publications

Species	Application	Title
mouse,human	WB,IF,IP	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang
human	WB,CoIP	Cancer Lett; ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer; Authors - Chen Zhou
mouse	WB	J Nanobiotechnology; Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles.; Authors - Ruijie Qian
human	IF	iScience; WASH interacts with Ku to regulate DNA double-stranded break repair.; Authors - Tao Wang
human	IF	Cell Rep; circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells; Authors - Qidong Li