KYNU Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86555-3-RR (same clone as 86555-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86555-3-RR (KYNU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86555-3-PBS in a different storage buffer formulation.

KYNU Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86555-3-PBS

Clone No.251437D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:3.7.1.3, Kynureninase, L kynurenine hydrolase, L-kynurenine hydrolase

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Product Information

86555-3-PBS targets KYNU in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2365

  Product name: Recombinant human KYNU protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-307 aa of BC000879

  Sequence: MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPARSEFFN

• Full Name: kynureninase (L-kynurenine hydrolase)
• Calculated Molecular Weight: 465aa,52 kDa; 307aa,35 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC000879
• Gene Symbol: KYNU
• Gene ID (NCBI): 8942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16719
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755).