Kininogen 1 Recombinant monoclonal antibody, PBS Only

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  WB analysis of human plasma using 84175-5-RR (same clone as 84175-5-PBS)

  Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 84175-5-RR (same clone as 84175-5-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84175-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

Kininogen 1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84175-5-PBS

Clone No.241397C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin

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Product Information

84175-5-PBS targets Kininogen 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1162

  Product name: Recombinant Human Kininogen-1 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-10*His

  Domain: 19-644 aa of NM_001102416.3

  Sequence: QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTVSPPHTSMAPAQDEERDSGKEQGHTRRHDWGHEKQRKHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNGKHNGWKTEHLASSSEDSTTPSAQTQEKTEGPTPIPSLAKPGVTVTFSDFQDSDLIATMMPPISPAPIQSDDDWIPDIQIDPNGLSFNPISDFPDTTSPKCPGRPWKSVSEINPTTQMKESYYFDLTDGLS

• Full Name: kininogen 1
• Calculated Molecular Weight: 72kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001102416.3
• Gene Symbol: Kininogen 1
• Gene ID (NCBI): 3827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01042-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.