L3MBTL3 Polyclonal antibody, PBS Only

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• L3MBTL3 Polyclonal antibody, PBS Only (28112-1-PBS)

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  WB analysis using 28112-1-AP (same clone as 28112-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 28112-1-AP (same clone as 28112-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

L3MBTL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28112-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

28112-1-PBS targets L3MBTL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27136

  Product name: Recombinant human L3MBTL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 524-646 aa of BC060845

  Sequence: LMEASEHGGCSTPGCKGIGHFKRARHLGPHSAANCPYSEINLNKDRIFPDRLSGEMPPASPSFPRNKRTDANESSSSPEIRDQHADDVKEDFEERTESEMRTSHEARGAREEPTVQQAQRRSA

• Full Name: l(3)mbt-like 3 (Drosophila)
• Calculated Molecular Weight: 88 aa, 3 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC060845
• Gene Symbol: L3MBTL3
• Gene ID (NCBI): 84456
• RRID: AB_2918148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.