LAG-3/CD223 Recombinant monoclonal antibody, PBS Only

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  WB analysis of CTLL-2 using 80867-1-RR (same clone as 80867-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80867-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80867-1-RR against Human LAG-3/CD223 were performed. The affinity constant is 8.47 nM.

LAG-3/CD223 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 80867-1-PBS

Clone No.1F14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

LAG-3, CD223, 1F14, LAG3, LAG 3

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Product Information

80867-1-PBS targets LAG-3/CD223 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_002286
• Gene Symbol: LAG-3
• Gene ID (NCBI): 3902
• RRID: AB_2918917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18627
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)