LAG-3 Recombinant antibody, PBS Only

LAG-3 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F14

Cat no : 80867-1-PBS

Synonyms

CD223, FDC, LAG 3, LAG3, LAG-3, lymphocyte activation gene 3, Protein FDC

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Validation Data Gallery

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  WB analysis of CTLL-2 using 80867-1-RR (same clone as 80867-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80867-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80867-1-PBS targets LAG-3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: fusion protein
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_002286
• Gene Symbol: LAG3
• Gene ID (NCBI): 3902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)