Mouse LAG-3/CD223 Recombinant monoclonal antibody, PBS Only

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  IHC staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using LAG-3/CD223 antibody (83941-7-RR, Clone: 241007B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using LAG-3/CD223 antibody (83941-7-RR, Clone: 241007B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83941-7-PBS

  Biolayer interferometry (BLl) kinetic assay of 83941-7-PBS against Mouse LAG-3/CD223 was performed. The affinity constant is 61.3 nM.

LAG-3/CD223 Uni-rAb^® Recombinant Antibody for IHC, Indirect ELISA

Cat No. 83941-7-PBS

Clone No.241007B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, Indirect ELISA

Synonyms

CD223, LAG-3, LAG 3, Lag3, Ly66

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Product Information

83941-7-PBS targets LAG-3/CD223 in IHC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1220

  Product name: Recombinant Mouse LAG-3/CD223 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 24-442 aa of NM_008479.2

  Sequence: GPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHL

• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: NM_008479.2
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q61790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)