Anti-Human LAIR1 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98326-1-RR

  1x10^6 human PBMCs were surface stained with 0.13 ug Anti-Human LAIR1 Rabbit RecAb (98326-1-RR, Clone:241805G2) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98326-1-RR

  1x10^6 human PBMCs were surface stained with 0.13 ug Anti-Human LAIR1 Rabbit RecAb (98326-1-RR, Clone:241805G2) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

LAIR1 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98326-1-RR

Clone No.241805G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98326-1-RR targets LAIR1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: leukocyte-associated immunoglobulin-like receptor 1
• Calculated Molecular Weight: 287 aa, 31 kDa
• GenBank Accession Number: BC027899
• Gene Symbol: LAIR1
• Gene ID (NCBI): 3903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6GTX8
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).