Tested Applications
| Positive WB detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26795-1-AP targets LAMB3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25192 Product name: Recombinant human LAMB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 828-1172 aa of BC075838 Sequence: AFLMAGQVAEQLRGFNAQLQRTRQMIRAAEESASQIQSSAQRLETQVSASRSQMEEDVRRTRLLIQQVRDFLTDPDTDAATIQEVSEAVLALWLPTDSATVLQKMNEIQAIAARLPNVDLVLSQTKQDIARARRLQAEAEEARSRAHAVEGQVEDVVGNLRQGTVALQEAQDTMQGTSRSLRLIQDRVAEVQQVLRPAEKLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVEQIRDHINGRVLYYATCK Predict reactive species |
| Full Name | laminin, beta 3 |
| Calculated Molecular Weight | 1172 aa, 130 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC075838 |
| Gene Symbol | LAMB3 |
| Gene ID (NCBI) | 3914 |
| RRID | AB_2880638 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13751 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMB3 is a protein that encodes the β3 chain of laminin; it is primarily involved in the formation of laminin 332 and constitutes a key component of the basal membrane in skin and mucosal epithelia. This protein is found at the junction between the epidermis and dermis, where it facilitates the adhesion and migration of epithelial cells as well as the establishment of the epidermal barrier, thereby maintaining the integrity of the skin structure. Mutations that impair the function of the LAMB3 gene can disrupt the connections in the basal membrane, leading to dystrophic epidermolysis bullosa, a condition characterized by blisters and erosions in the skin. In the field of oncology, abnormally high expression of LAMB3 can alter the extracellular matrix, promote epithelial-mesenchymal transition, and enhance the invasiveness and metastatic capacity of cancer cells; it often serves as a marker of poor prognosis in melanoma, lung cancer, and gastrointestinal tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LAMB3 antibody 26795-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Front Oncol MiR-24-3p Inhibits the Progression of Pancreatic Ductal Adenocarcinoma Through LAMB3 Downregulation. | ||
FASEB J Senolytic therapy ameliorates renal fibrosis postacute kidney injury by alleviating renal senescence. | ||
Int J Mol Sci Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients. | ||
Mol Med Rep Laminin‑332 mediates proliferation, apoptosis, invasion, migration and epithelial‑to‑mesenchymal transition in pancreatic ductal adenocarcinoma.
| ||
Biochem Pharmacol The LAMB3-EGFR signaling pathway mediates synergistic Anti-Cancer effects of berberine and emodin in Pancreatic cancer | ||
Br J Cancer LAMB3 drives gastric cancer progression through SAMD4A-mediated degradation of PHLPP2 mRNA leading to sustained PI3K-Akt activation. |
