LAMC3-Specific Monoclonal antibody

• 

  WB analysis of human testis using 67261-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 67261-1-Ig

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67261-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67261-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  Affinity and Kinetic Characterization of 67261-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 67261-1-PBS against Human GRP78/BIP were performed. The affinity constant is 0.559 nM.

LAMC3-Specific Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 67261-1-Ig

Clone No.4B8C5

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

LAMC3, 4B8C5, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma

---

Tested Applications

• Positive WB detected in: human testis tissue, mouse placenta tissue, PC-3 cells
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67261-1-Ig targets LAMC3-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19568

  Product name: Recombinant human LAMC3-Specific protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1225-1575 aa of NM_006059

  Sequence: VAEVLPEAESVLATVQQVGADTAPYLALLASPGALPQKSRAEDLGLKAKALEKTVASWQHMATEAARTLQTAAQATLRQTEPLTKLHQEARAALTQASSSVQAATVTVMGARTLLADLEGMKLQFPRPKDQAALQRKADSVSDRLLADTRKKTKQAERMLGNAAPLSSSAKKKGREAEVLAKDSAKLAKALLRERKQAHRRASRLTSQTQATLQQASQQVLASEARRQELEEAERVGAGLSEMEQQIRESRISLEKDIETLSELLARLGSLDTHQAPAQALNETQWALERLRLQLGSPGSLQRKLSLLEQESQQQELQIQGFESDLAEIRADKQNLEAILHSLPENCASWQ

• Full Name: laminin, gamma 3
• Calculated Molecular Weight: 1575 aa, 171 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: NM_006059
• Gene Symbol: LAMC3
• Gene ID (NCBI): 10319
• RRID: AB_2882532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6N6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; Laminin β4 is required for the development of human peripheral sensory neurons; Authors - Kenyi Saito-Diaz