LAMC3-Specific Monoclonal antibody

LAMC3-Specific Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4B8C5

Cat no : 67261-1-Ig

Synonyms

DKFZp434E202, LAMC3, LAMC3-Specific, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma, Laminin subunit gamma 3, laminin, gamma 3

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Validation Data Gallery

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  WB analysis of human testis using 67261-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 67261-1-Ig

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67261-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67261-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse placenta tissue, PC-3 cells
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67261-1-Ig targets LAMC3-Specific in WB, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LAMC3-Specific fusion protein Ag19568
• Full Name: laminin, gamma 3
• Calculated Molecular Weight: 1575 aa, 171 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: NM_006059
• Gene Symbol: LAMC3
• Gene ID (NCBI): 10319
• RRID: AB_2882532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.