LAMC3-Specific Monoclonal antibody

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• LAMC3-Specific Monoclonal antibody (67261-1-Ig)

LAMC3-Specific Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 67261-1-Ig Copied!

Clone No. 4B8C5

Publications (2)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

LAMC3, 4B8C5, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma

• 

  WB analysis of human testis using 67261-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse placenta using 67261-1-Ig

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 67261-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using 67261-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 67261-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 67261-1-PBS against Human GRP78/BIP were performed. The affinity constant is 0.559 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, mouse placenta tissue, PC-3 cells
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67261-1-Ig targets LAMC3-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19568

  Product name: Recombinant human LAMC3-Specific protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1225-1575 aa of NM_006059

  Sequence: VAEVLPEAESVLATVQQVGADTAPYLALLASPGALPQKSRAEDLGLKAKALEKTVASWQHMATEAARTLQTAAQATLRQTEPLTKLHQEARAALTQASSSVQAATVTVMGARTLLADLEGMKLQFPRPKDQAALQRKADSVSDRLLADTRKKTKQAERMLGNAAPLSSSAKKKGREAEVLAKDSAKLAKALLRERKQAHRRASRLTSQTQATLQQASQQVLASEARRQELEEAERVGAGLSEMEQQIRESRISLEKDIETLSELLARLGSLDTHQAPAQALNETQWALERLRLQLGSPGSLQRKLSLLEQESQQQELQIQGFESDLAEIRADKQNLEAILHSLPENCASWQ

• Full Name: laminin, gamma 3
• Calculated Molecular Weight: 1575 aa, 171 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: NM_006059
• Gene Symbol: LAMC3
• Gene ID (NCBI): 10319
• RRID: AB_2882532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6N6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody Laminin β4 (catalog no. 67261-1-Ig) has 2 total citations. Both appear in bioRxiv and Development, authored by Saito-Diaz et al., investigating the role of Laminin β4 in human peripheral sensory neuron development. The research field is neurodevelopment, and the application used was Western Blot (WB) in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; Laminin β4 is required for the development of human peripheral sensory neurons; Authors - Kenyi Saito-Diaz
human	WB	Development; Laminin β4 is required for the development of human peripheral sensory neurons.; Authors - Kenyi Saito-Diaz