CD107b / LAMP2 Recombinant monoclonal antibody

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  IF Staining of NIH/3T3 using 84474-7-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-7-RR, Clone: 241893C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84474-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84474-7-RR against Mouse CD107b / LAMP2 were performed. The affinity constant is below 1 pM.

CD107b / LAMP2 Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 84474-7-RR

Clone No.241893C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Synonyms

CD107b, Lamp2, Mac3, Lamp 2, 241893C1

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84474-7-RR targets CD107b / LAMP2 in IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: NM_001017959.2
• Gene Symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P17047-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.