CD107b / LAMP2 Polyclonal antibody

CD107b / LAMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27823-1-AP

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, pig liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27823-1-AP targets CD107b / LAMP2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD107b / LAMP2 fusion protein Ag27183
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Protocols

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 27823-1-APDownload protocol
IHC protocol for CD107b / LAMP2 antibody 27823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
mouseWB

Autophagy

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Authors - Xibao Zhao
pigIF

Autophagy

Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture

Authors - Tao Wang
mouseIF

J Neurosci

Effect of Aβ oligomers on neuronal APP triggers a vicious cycle leading to the propagation of synaptic plasticity alterations to healthy neurons.

Authors - Marta Rolland
mouseIF

J Neuroinflammation

Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.

Authors - Hanhai Zeng
mouseWB,IF

Front Cell Dev Biol

Differential Expression Profiles and Potential Intergenerational Functions of tRNA-Derived Small RNAs in Mice After Cadmium Exposure.

Authors - Ling Zeng