LAMP2 Polyclonal antibody

LAMP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27823-1-AP

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, pig liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27823-1-AP targets LAMP2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMP2 fusion protein Ag27183
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Protocols

Product Specific Protocols
WB protocol for LAMP2 antibody 27823-1-APDownload protocol
IHC protocol for LAMP2 antibody 27823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Cells

Autophagy Attenuation Hampers Progesterone Synthesis during the Development of Pregnant Corpus Luteum.

Authors - Zonghao Tang
humanWB

Oxid Med Cell Longev

Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration.

Authors - Liang Kang
ratWB

Cell Death Dis

PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming.

Authors - Wen-Qi Ma
mouseWB

J Mol Neurosci

PMID: 31243684

Authors - Yi Zhong
humanWB

Int J Biochem Cell Biol

Resveratrol induces depletion of TRAF6 and suppresses prostate cancer cell proliferation and migration.

Authors - Farjana Yeasmin Khusbu
humanWB

Life Sci

Clusterin contributes to hepatitis C virus-related hepatocellular carcinoma by regulating autophagy.

Authors - Na Fu