CD107b / LAMP2 Polyclonal antibody

CD107b / LAMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 27823-1-AP

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Validation Data Gallery

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  WB analysis using 27823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27823-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 27823-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, pig liver tissue, human placenta tissue
• Positive IHC detected in: human liver tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 15 publications below

Product Information

27823-1-AP targets CD107b / LAMP2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD107b / LAMP2 fusion protein Ag27183
• Full Name: lysosomal-associated membrane protein 2
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 120 kDa
• GenBank accession number: BC002965
• Gene symbol: LAMP2
• Gene ID (NCBI): 3920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
pig	IF	Autophagy; Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture; Authors - Tao Wang
mouse	WB	J Cell Physiol; Neuro death through autophagy via the acetylation of FoxO1 by SIRT2 in the hippocampus of mice in a autism spectrum disorder mice model; Authors - Lanmin Guo
mouse	IF	J Neurosci; Effect of Aβ oligomers on neuronal APP triggers a vicious cycle leading to the propagation of synaptic plasticity alterations to healthy neurons.; Authors - Marta Rolland
mouse	IF	J Neuroinflammation; Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.; Authors - Hanhai Zeng