LAMR1,RPSA Monoclonal antibody, PBS Only

LAMR1,RPSA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A9A1

Cat no : 67324-1-PBS

Synonyms

37/67 kDa laminin receptor, 37LRP, 40S ribosomal protein SA, 67 kDa laminin receptor, 67LR, LAMBR, Laminin receptor, Laminin receptor 1, LamR, LAMR1, LAMR1,RPSA, LBP/p40, LRP, LRP/LR, NEM/1CHD4, p40, ribosomal protein SA, RPSA

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Validation Data Gallery

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  WB analysis using 67324-1-Ig (same clone as 67324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67324-1-Ig (same clone as 67324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67324-1-Ig (same clone as 67324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67324-1-Ig (same clone as 67324-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1,RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67324-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67324-1-PBS targets LAMR1,RPSA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: LAMR1,RPSA fusion protein Ag6190
• Full Name: ribosomal protein SA
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC050688
• Gene Symbol: RPSA
• Gene ID (NCBI): 3921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.