Tested Applications
| Positive WB detected in | mouse lymph tissue, rat lymph tissue |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18895-1-AP targets LAPTM4B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13521 Product name: Recombinant human LAPTM4B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 31-317 aa of BC014129 Sequence: TDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA Predict reactive species |
| Full Name | lysosomal protein transmembrane 4 beta |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC014129 |
| Gene Symbol | LAPTM4B |
| Gene ID (NCBI) | 55353 |
| RRID | AB_10597385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VI4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LAPTM4B antibody 18895-1-AP | Download protocol |
| WB protocol for LAPTM4B antibody 18895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LAPTM4B Polyclonal antibody (Cat# 18895-1-AP) has 8 citations. Journals include Cell, Nature Communications, PNAS, Journal of Animal Science and Biotechnology, Frontiers in Cell and Developmental Biology, Cancer Management and Research, Biochemical and Biophysical Research Communications, and Frontiers in Oncology. Research fields span autophagy, mTORC1 signaling, EGF receptor function, and cancer biology—including hepatocellular, gastric, ovarian, and breast cancers—as well as EMT and copper-induced endoplasmic reticulum–lysosome regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation. | ||
J Anim Sci Biotechnol LAPTM4B as a key regulator in the copper-induced endoplasmic reticulum-lysosome interplay disorder in duck liver and the protective role of baicalin | ||
Front Cell Dev Biol Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific. | ||
Cancer Manag Res LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma. |
