LAPTM4B Polyclonal antibody

• Featured Product
• KD/KO Validated

LAPTM4B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18895-1-AP

Synonyms

LAPTM4B, LAPTM4beta, LC27

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Validation Data Gallery

• 

  WB analysis of mouse lymph using 18895-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 18895-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lymph tissue, rat lymph tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

18895-1-AP targets LAPTM4B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAPTM4B fusion protein Ag13521
• Full Name: lysosomal protein transmembrane 4 beta
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC014129
• Gene Symbol: LAPTM4B
• Gene ID (NCBI): 55353
• RRID: AB_10597385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.

Publications

Species	Application	Title
human	WB, IF	Cell; A kinase-independent role for EGF receptor in autophagy initiation.; Authors - Xiaojun Tan ; KD Validated
	WB	Nat Commun; LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.; Authors - Ruth Milkereit
human	IF	Proc Natl Acad Sci U S A; PtdIns(4,5)P2 signaling regulates ATG14 and autophagy.; Authors - Xiaojun Tan
	IHC	Front Cell Dev Biol; Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.; Authors - Xiaolei Yao
human	WB,IHC,IF	Biochem Biophys Res Commun; Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B.; Authors - Shengfei Zhou ; KD Validated
human	IF	Cancer Manag Res; LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma.; Authors - Fei Wang