LARG Polyclonal antibody

• Featured Product
• KD/KO Validated

LARG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 22441-1-AP

Synonyms

ARHGEF12, Leukemia-associated RhoGEF, Leukemia associated RhoGEF, KIAA0382

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Validation Data Gallery

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  WB analysis of Jurkat using 22441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 22441-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 22441-1-AP

  Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

22441-1-AP targets LARG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARG fusion protein Ag18211
• Full Name: Rho guanine nucleotide exchange factor (GEF) 12
• Calculated Molecular Weight: 1544 aa, 173 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC063117
• Gene Symbol: LARG
• Gene ID (NCBI): 23365
• RRID: AB_2879107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases.; Authors - Geng-Xian Shi
human	WB	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB,IHC	Am J Pathol; The Guanine Nucleotide Exchange Factor, Leukemia-Associated Rho Guanine Nucleotide Exchange Factor, and RhoC Play a Role in Glioblastoma Cell Invasion and Resistance.; Authors - Zonghui Ding ; KD Validated
mouse	WB	Int J Mol Sci; Inhibition of High-Temperature Requirement Protein A2 Protease Activity Represses Myogenic Differentiation via UPRmt; Authors - Hongyu Sun
mouse	WB,IP	J Neurosci; Ogt Deficiency Induces Abnormal Cerebellar Function and Behavioral Deficits of Adult Mice through Modulating RhoA/ROCK Signaling; Authors - Jinyu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs

LARG Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:22441-1-AP)