Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17277-1-AP targets LARP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11043 Product name: Recombinant human LARP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC030516 Sequence: MDSRDHGPGTSSVSTSNASPSEGAPLAGSYGCTPHSFPKFQHPSHELLKENGFTQQVYHKYRRRCLSERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFRQLAWEDAKENYRYGLECLFRFYSYGLEKKFRREIFQDFQEETKKDYESGQLYGLEKFWAYLKYSQSKTQSIDPKLQEYLCSFKRLEDFRVDEADPIE Predict reactive species |
| Full Name | La ribonucleoprotein domain family, member 2 |
| Calculated Molecular Weight | 201 aa, 24 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC030516 |
| Gene Symbol | LARP2 |
| Gene ID (NCBI) | 55132 |
| RRID | AB_2918052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q659C4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The La-related proteins (LARPs) share a conserved RNA-binding unit composed of a La Motif and an RNA Recognition Motif (RRM), which recognizes diverse RNA substrates. LARP2 has nine isoforms with MW 30-40 kDa, 90-105 kDa. Alternative splicing has been observed at this locus and multiple variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. (PMID: 26501340, PMID: 33292040)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LARP2 antibody 17277-1-AP | Download protocol |
| WB protocol for LARP2 antibody 17277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LARP2 Polyclonal antibody (Cat# 17277-1-AP) has 1 total citation. It appears in Translational Oncology (Impact Factor 4.9), published in 2024. The cited study investigated LARP1B induction under endoplasmic reticulum stress and its regulatory role in esophageal squamous cell carcinoma proliferation, placing the research field in oncology and cellular stress response. The application used was IHC on human samples.
| Species | Application | Title |
|---|---|---|
Transl Oncol Induction of LARP1B under endoplasmic reticulum stress and its regulatory role in proliferation of esophageal squamous cell carcinoma |
