LARP4 Polyclonal antibody

LARP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16529-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

La related protein 4, LARP4, PP13296

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of Y79 using 16529-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Y79 using 16529-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Y79 using 16529-1-AP
IP result of anti-LARP4 (IP:16529-1-AP, 4ug; Detection:16529-1-AP 1:300) with Y79 cells lysate 2000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 16529-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Y79 cells
Positive IP detected in	Y79 cells
Positive IHC detected in	human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16529-1-AP targets LARP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9777 Product name: Recombinant human LARP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC022377 Sequence: MYSSSCETTRNTTGIEESTDGMILGPEDLSYQIYDVSGESNSAVSTEDLKECLKKQLEFCFSRENLSKDLYLISQMDSDQFIPIWTVANMEEIKKLTTDPDLILEVLRSSPMVQVDEKGEKVRPSHKRCIVILREIPETTPIEEVKGLFKSENCPKVISCEFAHNSNWYITFQSDTDAQQAFILKRRS Predict reactive species
Full Name	La ribonucleoprotein domain family, member 4
Calculated Molecular Weight	188 aa, 21 kDa, 81 kDa
Observed Molecular Weight	81 kDa
GenBank Accession Number	BC022377
Gene Symbol	LARP4
Gene ID (NCBI)	113251
RRID	AB_2296671
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q71RC2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for LARP4 antibody 16529-1-AP	Download protocol
IP protocol for LARP4 antibody 16529-1-AP	Download protocol
WB protocol for LARP4 antibody 16529-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LARP4 antibody (Cat# 16529-1-AP) has 4 total citations published in Nucleic Acids Research, Frontiers in Cell and Developmental Biology, Journal of Molecular Biology, and Veterinary Microbiology. Associated research fields include RNA–protein interaction mapping in live cells, mechanosensing and cell migration regulation, structural biology of protein–protein interactions, and viral translation mechanisms of coronavirus PEDV.

Species	Application	Title
human	IP	Nucleic Acids Res SHIFTR enables the unbiased identification of proteins bound to specific RNA regions in live cells Authors - Jens Aydin View Article
human,mouse	WB,IF,IP	Front Cell Dev Biol Interaction of LARP4 to filamin A mechanosensing domain regulates cell migrations Authors - Zhenfeng Mao View Article
	WB	J Mol Biol Structural Basis of the LARP4-Filamin A Interaction and Competition with Integrin β7 Tails Authors - Zhenfeng Mao KD Validated View Article
human	WB,IF	Vet Microbiol Cellular RNA-binding proteins LARP4 and PABPC1 synergistically facilitate viral translation of coronavirus PEDV Authors - Jing Wang KD Validated View Article