LARP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

LARP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 17067-1-AP

Synonyms

P-TEFb-interaction protein for 7SK stability, PIP7S, La-related protein 7, La related protein 7, hLARP7

---

Validation Data Gallery

• 

  WB analysis using 17067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17067-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17067-1-AP (LARP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human spleen tissue, human stomach cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

17067-1-AP targets LARP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARP7 fusion protein Ag10891
• Full Name: La ribonucleoprotein domain family, member 7
• Calculated Molecular Weight: 582 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC006981
• Gene Symbol: LARP7
• Gene ID (NCBI): 51574
• RRID: AB_2132693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. binds to and stabilizes 7sk snRNA[PMID:19416841].

Publications

Species	Application	Title
mouse	WB	Genes Dev; Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.; Authors - Daiji Okamura
mouse,human	IF,IP	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji ; KD Validated
human	WB	Nucleic Acids Res; 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.; Authors - Eilebrecht Sebastian S
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB,IF,IP	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Monika (Verified Customer) (10-09-2023)

Great antibody, successfully used in western blots with 1:1000 to 1:2000 dilutions. Verified using a Calu-3 and HEK293T LARP7 knock-out cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung carcinoma, Calu-3 cells

LARP7 Antibody Western Blot validation (1:1000 dilution) in lung carcinoma, Calu-3 cells (Cat no:17067-1-AP)