LARS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

LARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 17097-1-AP

Synonyms

LeuRS, Leucyl-tRNA synthetase, Leucine--tRNA ligase, mitochondrial, LARS 2, KIAA0028

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Validation Data Gallery

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  WB analysis using 17097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17097-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17097-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17097-1-AP

  IP result of anti-LARS2 (IP:17097-1-AP, 4ug; Detection:17097-1-AP 1:500) with Jurkat cells lysate 2000ug.

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  IHC staining of human colon cancer using 17097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17097-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17097-1-AP (LARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

17097-1-AP targets LARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARS2 fusion protein Ag10576
• Full Name: leucyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 903 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC025989
• Gene Symbol: LARS2
• Gene ID (NCBI): 23395
• RRID: AB_2132807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family.

Publications

Species	Application	Title
mouse	IF	Circulation; Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells.; Authors - David T Paik
human	IF, WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	IF	EMBO Mol Med; The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.; Authors - Elena Perli
human,mouse	WB	Oxid Med Cell Longev; LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells.; Authors - Shujun Feng ; KD Validated
human	WB	J Biol Chem; Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.; Authors - Shasha Gong