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LARS2 Polyclonal antibody

LARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17097-1-AP

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:6.1.1.4, KIAA0028, LARS 2, Leucine--tRNA ligase, mitochondrial, Leucyl-tRNA synthetase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 17097-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 17097-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 17097-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 17097-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Jurkat using 17097-1-AP
IP result of anti-LARS2 (IP:17097-1-AP, 4ug; Detection:17097-1-AP 1:500) with Jurkat cells lysate 2000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 17097-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 17097-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 17097-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17097-1-AP (LARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17097-1-AP targets LARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10576 Product name: Recombinant human LARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 554-903 aa of BC025989 Sequence: VDLYIGGKEHAVMHLFYARFFSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLALVPRKLCAHYTWDASVLLQAWPAVDPEFLQQPEVVQMAVLINNKACGKIPVPQQVARDQDKVHEFVLQSELGVRLLQGRSIKKSFLSPRTALINFLVQD Predict reactive species
Full Name	leucyl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight	903 aa, 102 kDa
Observed Molecular Weight	102 kDa
GenBank Accession Number	BC025989
Gene Symbol	LARS2
Gene ID (NCBI)	23395
RRID	AB_2132807
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15031
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family.

Protocols

Product Specific Protocols
IF protocol for LARS2 antibody 17097-1-AP	Download protocol
IHC protocol for LARS2 antibody 17097-1-AP	Download protocol
IP protocol for LARS2 antibody 17097-1-AP	Download protocol
WB protocol for LARS2 antibody 17097-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LARS2 Polyclonal antibody (Cat# 17097-1-AP) has 14 citations spanning journals including Cell, Circulation, Bone Research, Redox Biology, Molecular Cell, Nucleic Acids Research, EMBO Molecular Medicine, and others. Research fields cover lung stem cell biology, endothelial cell transcriptomics, fracture healing, diabetic muscle OXPHOS, mTORC1 signaling, mitochondrial biogenesis and hearing, renal fibrosis, mitochondrial tRNA mutations, ovarian granulosa cell apoptosis, retinal energy metabolism, and single-cell gene expression profiling.

Species	Application	Title
mouse	IF	Cell Tracing the origin of alveolar stem cells in lung repair and regeneration Authors - Kuo Liu View Article
mouse	IF	Circulation Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells. Authors - David T Paik View Article
		Bone Res Mechanism of Piezo1 regulating chondrocyte mitochondrial function and promoting fracture healing through β-catenin/LARS2 signaling pathway Authors - Tao Zhang View Article
mouse	WB	Redox Biol Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle Authors - Iliana López-Soldado View Article
human	WB,IF	Mol Cell Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. Authors - Sung-Hoon Kim View Article
zebrafish	WB	Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. Authors - Qinghai Zhang View Article