LBR Monoclonal antibody, PBS Only

LBR Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B7

Cat no : 68378-1-PBS

Synonyms

DHCR14B, FLJ43126, lamin B receptor, LBR, LMN2R, PHA

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Validation Data Gallery

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  WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68378-1-PBS targets LBR in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LBR fusion protein Ag25601
• Full Name: lamin B receptor
• Calculated Molecular Weight: 615 aa, 71 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC020079
• Gene Symbol: LBR
• Gene ID (NCBI): 3930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).