LBR Recombinant monoclonal antibody

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  IF Staining of HeLa using 84522-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 84522-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84522-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84522-7-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-7-RR, Clone:241864G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

LBR Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 84522-7-RR

Clone No.241864G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

14, 241864G10, 3-beta-hydroxysterol Delta, C-14 sterol reductase, C14SR

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84522-7-RR targets LBR in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3088

  Product name: Recombinant human LBR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of BC020079

  Sequence: MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFG

• Full Name: lamin B receptor
• Calculated Molecular Weight: 615 aa, 71 kDa
• GenBank Accession Number: BC020079
• Gene Symbol: LBR
• Gene ID (NCBI): 3930
• RRID: AB_3672030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14739
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533).