Tested Applications
| Positive WB detected in | mouse testis tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12243-1-AP targets LCAT in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2887 Product name: Recombinant human LCAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 94-440 aa of BC014781 Sequence: LGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE Predict reactive species |
| Full Name | lecithin-cholesterol acyltransferase |
| Calculated Molecular Weight | 440 aa, 50 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC014781 |
| Gene Symbol | LCAT |
| Gene ID (NCBI) | 3931 |
| RRID | AB_10638476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04180 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LCAT antibody 12243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LCAT Polyclonal antibody (Cat# 12243-1-AP) has 8 citations published in journals including Nature Communications, Lipids in Health and Disease, Food & Function, Investigative Ophthalmology & Visual Science, ACS Chemical Neuroscience, Cell Physiology & Biochemistry, Heliyon, and bioRxiv. Research fields span lipid/cholesterol metabolism, atherosclerosis, hepatosteatosis, glaucomatous optic nerve pathology, CNS demyelination/remyelination, and hyperlipidemia.
| Species | Application | Title |
|---|---|---|
Nat Commun Sex-specific KDM6A-HNF4A-CREBH network controls lipoprotein cholesterol metabolism and atherosclerosis via epigenetic reprograming of hepatocytes. | ||
Lipids Health Dis Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats. | ||
Food Funct Dual improvement effects of ω-3 on bone loss and liver injury in rats with liver-bone axis damage. | ||
Invest Ophthalmol Vis Sci Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves. | ||
ACS Chem Neurosci Temporal Dynamics of CNS Cholesterol Esters Correlate with Demyelination and Remyelination. | ||
Cell Physiol Biochem Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. |
