LCAT Polyclonal antibody

LCAT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12243-1-AP

Synonyms

LCAT

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12243-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12243-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12243-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12243-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 12243-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12243-1-AP targets LCAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCAT fusion protein Ag2887
• Full Name: lecithin-cholesterol acyltransferase
• Calculated Molecular Weight: 440 aa, 50 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014781
• Gene Symbol: LCAT
• Gene ID (NCBI): 3931
• RRID: AB_10638476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse		Cell Physiol Biochem; Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.; Authors - Dandan Zhong
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck
rat	WB	Lipids Health Dis; Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats.; Authors - Shuling Wang