LCAT Recombinant monoclonal antibody

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  WB analysis using 86041-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86041-1-RR (LCAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 86041-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86041-1-RR (LCAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86041-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86041-1-RR (LCAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LCAT Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86041-1-RR

Clone No.250320E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lecithin-cholesterol acyltransferase, PAF acetylhydrolase

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Tested Applications

• Positive WB detected in: mouse blood, rat plasma
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86041-1-RR targets LCAT in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2756

  Product name: Recombinant Mouse LCAT protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 25-438 aa of NM_008490.2

  Sequence: FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAPHVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLGAYRTPKSPAASPSPPPPE

• Full Name: lecithin cholesterol acyltransferase
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: NM_008490.2
• Gene Symbol: Lcat
• Gene ID (NCBI): 16816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16301
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).