LCK Polyclonal antibody

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  WB analysis of Jurkat using 12477-1-AP

  WB result of LCK antibody (12477-1-AP, 1:2000) with si-control and si-LCK transfected Jurkat cells.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12477-1-AP

  IP result of anti-LCK (IP:12477-1-AP, 4ug; Detection:12477-1-AP 1:500) with Jurkat cells lysate 4000ug.

• Featured Product
• KD/KO Validated

LCK Polyclonal Antibody for WB, IP, ELISA

Cat No. 12477-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells, Ramos cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

12477-1-AP targets LCK in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3176

  Product name: Recombinant human LCK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 236-539 aa of BC013200

  Sequence: DEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLPTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

• Full Name: lymphocyte-specific protein tyrosine kinase
• Calculated Molecular Weight: 539 aa, 56 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC013200
• Gene Symbol: LCK
• Gene ID (NCBI): 3932
• RRID: AB_2136316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06239
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Ovarian Res; Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors.; Authors - Katie K Crean-Tate
mouse	WB	Front Pharmacol; "Drying effect" of fructus aurantii components and the mechanism of action based on network pharmacology and in vitro pharmacodynamic validation; Authors - Jing Zhu
human	WB	J Ovarian Res; LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer; Authors - Goutam Dey
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang
mouse	WB	Pathol Res Pract; Asiaticoside enhances the anti-tumor effect of anti-PDL1 by regulating T cell activity through increasing LCK activity; Authors - Qingyi Ren