LCK Polyclonal antibody

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• LCK Polyclonal antibody (32464-1-AP)

LCK Polyclonal Antibody for WB, ELISA

Cat No. 32464-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, FC, ELISA

Synonyms

p56-LCK, Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56

• 

  WB analysis using 32464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32464-1-AP (LCK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, Ramos cells, mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32464-1-AP targets LCK in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38061

  Product name: Recombinant human LCK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-240 aa of BC013200

  Sequence: MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEV

• Full Name: lymphocyte-specific protein tyrosine kinase
• Calculated Molecular Weight: 539 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC013200
• Gene Symbol: LCK
• Gene ID (NCBI): 3932
• RRID: AB_3742617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P06239
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Publications

What published studies show

The LCK Polyclonal antibody (Cat# 32464-1-AP) has 1 citation to date. It was cited in Cell Communications & Signaling (IF 11.6) for a study investigating p53-regulated early mucosal immunity via antigen presentation in zebrafish intestine following SVCV vaccination. The research field encompasses mucosal immunology, antigen presentation, and vaccine response in zebrafish models. The antibody was applied by flow cytometry in this publication.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
zebrafish	FC	Cell Commun Signal; p53 regulates early mucosal immunity via antigen presentation in zebrafish intestine post-SVCV vaccination.; Authors - Zhao Zhao